Postsynaptic actin regulates active zone spacing and glutamate receptor...
Synaptic communication requires precise alignment of presynaptic active zones with postsynaptic receptors to enable rapid and efficient neurotransmitter release. How transsynaptic signaling between...
View ArticleHistone H4 tail mediates allosteric regulation of nucleosome remodelling by...
Imitation switch (ISWI)-family remodelling enzymes regulate access to genomic DNA by mobilizing nucleosomes. These ATP-dependent chromatin remodellers promote heterochromatin formation and...
View ArticleA model for the generation and interconversion of ER morphologies.
The peripheral endoplasmic reticulum (ER) forms different morphologies composed of tubules and sheets. Proteins such as the reticulons shape the ER by stabilizing the high membrane curvature in...
View ArticleA PIK3C3-ankyrin-B-dynactin pathway promotes axonal growth and multiorganelle...
Axon growth requires long-range transport of organelles, but how these cargoes recruit their motors and how their traffic is regulated are not fully resolved. In this paper, we identify a new pathway...
View ArticleSingle-molecule super-resolution imaging of chromosomes and in situ haplotype...
Fluorescence in situ hybridization (FISH) is a powerful single-cell technique for studying nuclear structure and organization. Here we report two advances in FISH-based imaging. We first describe the...
View ArticleCorrelative stochastic optical reconstruction microscopy and electron...
Correlative fluorescence light microscopy and electron microscopy allows the imaging of spatial distributions of specific biomolecules in the context of cellular ultrastructure. Recent development of...
View ArticlePurinosome formation as a function of the cell cycle.
The de novo purine biosynthetic pathway relies on six enzymes to catalyze the conversion of phosphoribosylpyrophosphate to inosine 5'-monophosphate. Under purine-depleted conditions, these enzymes...
View ArticleRNA imaging. Spatially resolved, highly multiplexed RNA profiling in single...
Knowledge of the expression profile and spatial landscape of the transcriptome in individual cells is essential for understanding the rich repertoire of cellular behaviors. Here, we report multiplexed...
View ArticleHigh-performance probes for light and electron microscopy.
We describe an engineered family of highly antigenic molecules based on GFP-like fluorescent proteins. These molecules contain numerous copies of peptide epitopes and simultaneously bind IgG...
View ArticleMapping Synaptic Input Fields of Neurons with Super-Resolution Imaging.
As a basic functional unit in neural circuits, each neuron integrates input signals from hundreds to thousands of synapses. Knowledge of the synaptic input fields of individual neurons, including the...
View ArticleChromatin topology is coupled to Polycomb group protein subnuclear organization.
The genomes of metazoa are organized at multiple scales. Many proteins that regulate genome architecture, including Polycomb group (PcG) proteins, form subnuclear structures. Deciphering mechanistic...
View ArticleStepwise nucleosome translocation by RSC remodeling complexes.
The SWI/SNF-family remodelers regulate chromatin structure by coupling the free energy from ATP hydrolysis to the repositioning and restructuring of nucleosomes, but how the ATPase activity of these...
View ArticleAn RNA-aptamer-based two-color CRISPR labeling system.
The spatial organization and dynamics of chromatin play important roles in essential biological functions. However, direct visualization of endogenous genomic loci in living cells has proven to be...
View ArticleSpatial organization shapes the turnover of a bacterial transcriptome.
Spatial organization of the transcriptome has emerged as a powerful means for regulating the post-transcriptional fate of RNA in eukaryotes; however, whether prokaryotes use RNA spatial organization...
View ArticleSuper-resolution imaging reveals distinct chromatin folding for different...
Metazoan genomes are spatially organized at multiple scales, from packaging of DNA around individual nucleosomes to segregation of whole chromosomes into distinct territories. At the intermediate...
View ArticleSpatial colocalization and functional link of purinosomes with mitochondria.
Purine biosynthetic enzymes organize into dynamic cellular bodies called purinosomes. Little is known about the spatiotemporal control of these structures. Using super-resolution microscopy, we...
View ArticleReal-Time Imaging of Translation on Single mRNA Transcripts in Live Cells.
Translation is under tight spatial and temporal controls to ensure protein production in the right time and place in cells. Methods that allow real-time, high-resolution visualization of translation...
View ArticlePrevalent presence of periodic actin-spectrin-based membrane skeleton in a...
Actin, spectrin, and associated molecules form a periodic, submembrane cytoskeleton in the axons of neurons. For a better understanding of this membrane-associated periodic skeleton (MPS), it is...
View ArticleSpatial organization of chromatin domains and compartments in single...
The spatial organization of chromatin critically affects genome function. Recent chromosome-conformation-capture studies have revealed topologically associating domains (TADs) as a conserved feature...
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